Overview
Revvity offers two IVISbrite™ bioluminescent luciferase substrates for in vitro, in vivo and ex vivo bioluminescent imaging: D-luciferin and coelenterazine H. D-luciferin is a substrate for Firefly luciferase (such as Red-FLuc), and is used in conjunction with our IVISbrite Tumor Cell Lines or cells transfected with our IVISbrite Red F-luc-Puromycin (formerly RediFect™ Red-FLuc) and IVISbrite Red F-luc-GFP (formerly Red-FLuc-GFP) lentiviral particles for in vitro, in vivo or ex vivo imaging. Coelenterazine H is a substrate for Renilla and Gaussia luciferase and can be used for in vivo imaging with cells transfected with our IVISbrite Green Renilla-Puromycin (formerly RediFect Green RenLuc-Puromycin) lentiviral particles, or when either of these luciferases is used as a reporter. The respective substrate is necessary for bioluminescent visualization in vitro, in vivo or ex vivo.
After the bioluminescent cell line expressing firefly luciferase, Renilla luciferase, or Gaussia luciferase is established in a mouse or other small animal model, the luciferin or coelenterazine substrate is injected. The substrate is then used by the bioluminescent reporter to produce light within the cell line, which allows visualization of the bioluminescent cells within the mouse (or other) model. This luminescence can be visualized and measured using an appropriate 2D or 3D imager.
Products and catalog numbers
| Product | Catalog number | Substrate specificity | Quantity | Formula | Fluorescent Tracer* |
|---|---|---|---|---|---|
| IVISbrite D-Luciferin Potassium Salt Bioluminescent Substrate | 122799 | Firefly luciferase substrate | 1g (~200 (30g) mice) | Powder | N |
| IVISbrite D-Luciferin Bioluminescent Substrate - RediJect™ Solution | 770504 | Firefly luciferase substrate | 10 vials (5 (30g) mice/vial) | RediJect Solution | N |
| IVISbrite D-Luciferin Ultra Bioluminescent Substrate - RediJect Solution | 770505 | Firefly luciferase substrate | 10 vials (5 (30g) mice/vial) | RediJect Solution | Y |
| IVISbrite Coelenterazine h Bioluminescent Substrate - RediJect Solution | 760506 | Renilla or Gaussia luciferase substrate | 10 vials (5 (30g) mice/vial) | RediJect Solution | N |
*Pre-formulated with a rapidly clearing fluorescent dye to validate the integrity of your substrate injection, so with a quick fluorescent image on your imaging system you know immediately if an injection failed, and you can normalize your bioluminescence results
**RediJect products are supplied in a ready-to-use injectable format
Protocols
- Preparation of luciferin for in vitro or in vivo assays (can be used for Cat. No. 122796)
- Determining luciferin kinetic curve for your model
- Using Coelenterazine H for in vivo imaging
FAQs
Q. How do I know whether to use the intraperitoneal (i.p.) luciferin injection protocol or the intravenous (i.v.) injection protocol, when injecting luciferin for bioluminescence in vivo imaging?
A. Most scientists use i.p injections. Subcutaneous injections are sometimes performed as well. If the signal is weak, you can do an i.v. injection, but you will need to image as soon as you inject (which can be very cumbersome).
Q. What if I was using product number 122796, 119222, or XR-1001 previously?
A. These products have been replaced by product number 122799. They are all firefly D-luciferin, potassium salt formulations.
For research use only. Not for use in diagnostic procedures. The information provided above is solely for informational and research purposes only. The information does not constitute medical advice and must not be used or interpreted as such. Consult a qualified veterinarian or researcher for specific guidance or use information. Revvity assumes no liability or responsibility for any injuries, losses, or damages resulting from the use or misuse of the provided information, and Revvity assumes no liability for any outcomes resulting from the use or misuse of any recommendations. The information is provided on an "as is" basis without warranties of any kind. Users are responsible for determining the suitability of any recommendations for the user’s particular research. Any recommendations provided by Revvity should not be considered a substitute for a user’s own professional judgment. Users are solely responsible for complying with all relevant laws, regulations, and institutional animal care and use committee (IACUC) guidelines in their use of the information provided.