Alpha Assays

View products & services View resource library

Virtually any target can be quickly and easily measured using Alpha technology, an improved alternative to quantitative ELISA assays and ELISA reagents. Such targets include enzymes, receptor-ligand interactions, low-affinity interactions, second messenger levels, DNA, RNA, proteins, protein-protein interactions, peptides, sugars and small molecules.

Ease-of-use: homogeneous assay with no wash requirement and no separation steps
Fast: results in 3 hours for most applications
High sensitivity: detect down to femtogram levels of target
Large dynamic range: 4-5 logs
Compatible with many samples types: serum, plasma, cerebrospinal fluid (CSF), tissue extracts, cell culture supernatants, cell lysates, and more

Choose the homogenous assay right for you:

AlphaLISA^™ – when running large numbers of ELISA assays from the same sample or working with a difficult matrix, serum or plasma
AlphaLISA SureFire^® Ultra^™ – an excellent choice for studying pathways or phosphorylated proteins in whole cells, or studying endogenous receptors
AlphaScreen^® – perfect for studying fusion-tagged proteins or protein-protein interactions, and for testing cAMP, phosphorylated peptides or samples in a simple matrix
Alpha Toolbox – when your target doesn’t have an off-the-shelf quantitative ELISA assay, the toolbox gives you everything you need to create your own

For research use only. Not for use in diagnostic procedures.

Shop now

Block2-AlphaLisa-SureFire-Ultra-Phosphoprotein-Cell-Signaling

Biomarker/cytokine detection

Detection and quantitation of molecular targets in serum, plasma, culture supernatants, cellular lysates, and other sample types.

Convert troublesome traditional ELISAs to a far superior alternative - Revvity's highly sensitive, homogeneous AlphaLISA platform for biomarkers and cytokines. Our exclusive AlphaLISA assay platform offers a wide dynamic range and easy automation for analysis of biomarkers, cytokines and other analytes. With AlphaLISA you can also shorten the time it takes you to run assays to less than 3 hours and benefit from small sample volumes (1-5 µL) to conserve precious sample.

Cell signaling

AlphaLISA SureFire Ultra and Alpha SureFire Multiplex assay kits to measure protein phosphorylation in cell signaling pathways.

All-in-one-well alternative to Western Blotting
Flexibility to use cultured cell lines, stem cells and primary cells
Cost-effective and flexible – a single plate can accommodate any assay for any target
Great sensitivity and dynamic range
Easy automation
No harmful chemicals and non-radioactive, eliminating special reagent handling and disposal

Protein-protein interactions

Handle large biomolecular interactions with ease.

Ease-of-use: few addition steps, fast assay development
Broad range of affinities: detect strong or weak interactions, from pM to mM affinity
Distance: measure very large protein or antibody complexes – spanning up to 200 nm or more
High avidity: multiple binding sites on each bead enables use of nanomolar concentrations of antibodies or proteins, as well as use of low affinity binders
Conserve reagents: robust assays using nM concentrations of proteins/antibodies

Epigenetics

Epigenetics is the analysis of heritable traits that are not transmitted by sequence information but rather through modification of the DNA methylation state and Histone methylation/acetylation driven by environmental factors such as lifestyle, toxins and stress.

Revvity made available for epigenetics research a range of biochemical and cellular assays. AlphaLISA® epigenetics toolbox reagents were designed for the detection of specific methylation and acetylation marks on histone peptide substrates. AlphaLISA epigenetic cellular detection kits were designed to detect endogenous epigenetic modifications on Histone 3 in a no-wash, all-in-one well format. In addition to the acetylation and methylation assays mentioned above, other types of post-translational modification detection assays have been created using Alpha reagents to detect specific epigenetic marks on both peptide and full-length histone and protein substrates.